Coding

Part:BBa_K2699000:Design

Designed by: Bi-ru Amy Yeung   Group: iGEM18_Toronto   (2018-10-10)


Arg1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 863
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1482
    Illegal BamHI site found at 4887
    Illegal XhoI site found at 4575
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1933
    Illegal NgoMIV site found at 2335
    Illegal AgeI site found at 4854
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 969
    Illegal SapI.rc site found at 3745
    Illegal SapI.rc site found at 4501


Design Notes

There were no design considerations that had to be dealt with using this sequence


Source

This part was derived and constructed from the Shapiro lab, taking genomic sequences from B. megaterium and A. flos-aquae.

References